不同靈芝菌株菌絲體乙醇提取物體外抑瘤活性的比較和機(jī)理

A COMPARISON STUDY OF THE IN VITROANTICANCER ACTIVITY AND MECHANISM OF ETHANOL EXTRACTS FROM MYCELIA OF DIFFERENT GANODERMA LUCIDUM STRAINS

楊曉彤　 周月琴　 李緒全　 馮慧琴　 糜可　 楊慶堯　

摘　要：以人急性早幼粒白血病細(xì)胞HL-60細(xì)胞株作為模型,用MTT法測定生長抑制率,流式細(xì)胞Annexin V實(shí)驗(yàn)和DNA含量法研究抑瘤機(jī)制,比較了8個(gè)靈芝Ganodermalucidum菌株發(fā)酵菌絲體乙醇提取物的體外抗腫瘤活性和抑瘤機(jī)制.篩選結(jié)果得到了能產(chǎn)生高抑瘤活性乙醇提取物的靈芝菌株L5.其對HL-60細(xì)胞的抑制率為91.4±0.9%(72小時(shí),125μg/mL);作用48小時(shí)后,13.3%的細(xì)胞發(fā)生早期凋亡,G0/G1期細(xì)胞比例比對照組增加15.9%,而S期細(xì)胞比例則下降了8.4%,G2/M期細(xì)胞數(shù)減少7.6%.本研究證明了靈芝菌絲體乙醇提取物能有效抑制HL-60腫瘤細(xì)胞的體外生長,抑制率與菌株相關(guān),其抑瘤機(jī)制與細(xì)胞Go/G1期阻滯和誘導(dǎo)凋亡有關(guān).

關(guān)鍵詞：細(xì)胞周期,凋亡,HL-60細(xì)胞

分類號：Q936　文獻(xiàn)標(biāo)識碼：A

文章編號：1672-6472(2005)02-0251-0258

基金項(xiàng)目：上海市教育委員會(huì)科學(xué)項(xiàng)目資助(項(xiàng)目號04DB20)

作者簡介：楊曉彤,通訊作者:email:xtyang@shnu.edu.cn

作者單位：楊曉彤（上海師范大學(xué)微生物與免疫學(xué)研究所,上海,200234）　

　　　　　周月琴（上海師范大學(xué)微生物與免疫學(xué)研究所,上海,200234）　

　　　　　李緒全（上海師范大學(xué)微生物與免疫學(xué)研究所,上海,200234）　

　　　　　馮慧琴（上海師范大學(xué)微生物與免疫學(xué)研究所,上海,200234）　

　　　　　糜可（上海師范大學(xué)微生物與免疫學(xué)研究所,上海,200234）　

　　　　　楊慶堯（上海師范大學(xué)微生物與免疫學(xué)研究所,上海,200234）　

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2005 Vol.24 No.2 P.251-258