【作者】趙微微; 李海波; 付立忠; 吳學(xué)謙; 許修宏; 賀亮; 程俊文; 魏海龍; 吳慶其
【Author】ZHAO Weiwei1; LI Haibo 2; FU Lizhong2; 3; WU Xueqian 2; 3; XU Xiuhong1; HE Liang 2; CHENG Junwen 2; WEI Hailong2; 3; WU Qingqi2; 31Northeast Agricultural University; Harbin; Heilongjiang 150030; China; 2ZhejiangForestry Academy; Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of ForestResource; Hangzhou; Zhejiang 310023; China; 3Lishui Edible Fungal Research and DevelopmentCenter; Lishui; Zhejiang 323000; China
【作者機(jī)構(gòu)】東北農(nóng)業(yè)大學(xué); 浙江省林業(yè)科學(xué)研究院浙江省森林資源生物與化學(xué)利用重點(diǎn)實(shí)驗(yàn)室; 麗水市食用菌研究開(kāi)發(fā)中心
【摘要】 利用前期研究開(kāi)發(fā)的10個(gè)SCAR分子標(biāo)記對(duì)47個(gè)香菇主栽菌株的基因組DNA進(jìn)行擴(kuò)增分析?；赟CAR標(biāo)記在檢測(cè)菌株中的存在與缺失差異,7個(gè)香菇菌株被首先鑒別出來(lái),其余40個(gè)菌株被分成11個(gè)小組,其中一些形態(tài)特征極為相近的菌株,如L241與L241-4、閩豐1號(hào)與L12、Cr02與7402等被相互辨別。
【Abstract】 Forty-seven Lentinula edodes strains that are presently cultivated in China on a large scale were collected and analyzed with 10 previously developed stable sequence characterized amplified region(SCAR) molecular markers.Based on detected differences in SCAR phenotypes,seven individual L.edodes strains were identified,and the remaining 40 strains were separated into 11 distinct groups.Our data provide the first molecular-based evidence confirming the distinction between closely related L.edodes strains having similar morphological features,including strains L241 and L241-4,Minfeng-1 and L12,and Cr02 and 7402.
【關(guān)鍵詞】 香菇; 主栽品種; SCAR標(biāo)記; 分子鑒定; 食用菌
【Key words】 Lentinula edodes strains; major cultivars; SCAR molecular marker; molecular identification; edible mushroom
【基金】“十一五”國(guó)家科技支撐計(jì)劃課題“人工栽培食用菌優(yōu)質(zhì)新品種選育研究”(編號(hào):2008BADA1B02);; 浙江省創(chuàng)新團(tuán)隊(duì)建設(shè)與人才培養(yǎng)項(xiàng)目“現(xiàn)代食用菌技術(shù)創(chuàng)新團(tuán)隊(duì)建設(shè)”(編號(hào):2009F20019);; 浙江省農(nóng)業(yè)科技成果轉(zhuǎn)化資金項(xiàng)目“專(zhuān)用型香菇新品種推廣與種性安全快速鑒定技術(shù)示范應(yīng)用”的部分研究?jī)?nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-002
【分類(lèi)號(hào)】S646.12