柯野; 張小平; 譚偉; 丁海萍
廣東韶關(guān)學(xué)院生物工程系; 四川農(nóng)業(yè)大學(xué)資環(huán)學(xué)院; 四川省農(nóng)業(yè)科學(xué)院土壤肥料研究所; 四川農(nóng)業(yè)大學(xué)資環(huán)學(xué)院 廣東韶關(guān)512005; 四川農(nóng)業(yè)大學(xué)資環(huán)學(xué)院; 四川雅安625014 ; 四川雅安625014 ; 四川成都610066 ; 四川雅安625014

【中文摘要】 對(duì)峙培養(yǎng)試驗(yàn)結(jié)果表明 ,長(zhǎng)根金錢菌對(duì)玉米小斑病菌菌絲生長(zhǎng)有很強(qiáng)的拮抗作用。L16 (4 5)正交試驗(yàn)結(jié)果表明 ,以玉米粉 4 % ,蔗糖 3% ,黃豆粉 0 .5 % ,蛋白胨 0 .2 %為培養(yǎng)基 ,初始pH6時(shí) ,液體培養(yǎng)長(zhǎng)根金錢菌發(fā)酵液對(duì)玉米小斑病菌菌絲生長(zhǎng)的抑制作用最強(qiáng) ,抑制率與長(zhǎng)根金錢菌的生物量呈顯著正相關(guān)。發(fā)酵液中的粗蛋白對(duì)玉米小斑病菌菌絲生長(zhǎng)有極顯著的抑制作用 ,但粗多糖對(duì)玉米小斑病菌菌絲生長(zhǎng)沒有抑制作用

【英文摘要】 The significant antagonism of Oudemansiella radicata to Bipolaris maydis was found in the antagnistic test. And the results of L16(45)orthogonal test of O. radicata in submerged culture proved that the broth had the strongest antagonism to the mycelial growth of B. maydis, when the submerged-culture medium was composed of corn meal 4%, sucrose 3%, soybean meal 0.5% and peptone 0.2% with the initial pH being 6.0. Furthermore, there was a significant positive correlation between the mycelial biomass of O. radiacate and the inhibition rate of broth to the mycelial growth of B. maydis, and the crude protein in the broth significantly inhibited the mycelial growth of B. maydis, but the crude polysaccharide did not have such inhibition effect.

【中文關(guān)鍵詞】 長(zhǎng)根金錢菌; 玉米小斑病菌; 發(fā)酵液; 拮抗作用
【英文關(guān)鍵詞】 Oudemansiella radicata; Bipolaris maydis; Broth; Antagonism
【基金】國(guó)家"十五"科技攻關(guān)項(xiàng)目"攀西地區(qū)特色生物資源綜合開發(fā)與示范"(2001BA901A40)的部分內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2004年04期 【DOI】CNKI:SUN:SYJB.0.2004-04-006