裂褶菌產(chǎn)纖維二糖脫氫酶條件優(yōu)化及
部分酶學(xué)性質(zhì)研究
方靖 劉穩(wěn) 高培基
摘 要：研究了裂褶菌Schizophyllum communeAS 5.391產(chǎn)纖維二糖脫氫酶的條件。該酶是誘導(dǎo)酶，棉花是最好的誘導(dǎo)底物。培養(yǎng)基中加入琥珀酸鈉緩沖液和土溫80利于酶的合成。而木素相關(guān)物質(zhì)藜蘆醇或愈創(chuàng)木酚對(duì)酶的生成沒有影響。該酶在pH3.5～10.5和45℃以下保持穩(wěn)定，其最適作用溫度為30℃，最適pH為4.5。Zn2＋和Ag＋對(duì)該酶活性有很大的抑制作用，而疊氮化鈉和氰化鉀對(duì)酶活力沒有影響。
關(guān)鍵詞： 裂褶菌，纖維二糖脫氫酶，酶的合成，酶性質(zhì)研究
中圖分類號(hào)： Q939.97 文獻(xiàn)標(biāo)識(shí)碼： A
文章編號(hào)： 1007-3515（2000）01-0107-0110
OPTIMIZATION OF CELLOBIOSE DEHYDROGENASE
PRODUCTION BY SCHIZOPHYLLUM COMMUNE
AND SOME PROPERTIES OF THE ENZYME
FANG Jing, LIU Wen, GAO PeiJi
(The state Key Laboratory of Microbial Technology, Shandong University, Jinan 250100)
ABSTRACT: Effects of various parameters on cellobiose dehydrogenase (CDH) production by Schizophyllum commune AS 5.391 were determined. CDH was induced by cellulosic substrates, and cotton cellulose with high crystallinity supported the maximum CDH production. Addition of succinate buffer and Tween 80 facilitated CDH secretion. The optimized temperature and pH for CDH are 30℃ and 4.5. It keeps stable at pH range of 3.5～10.5, and below 45℃. CDH was inhibited by Zn2＋ and Ag＋, but it was not influenced by azide and cyanide.
KEY WORDS: Schizophyllum commune, Cellobiose Dehydrogenase, Enzyme Production, Characterization
國(guó)家自然科學(xué)基金（39430020）及山東大學(xué)微生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室資助項(xiàng)目
方靖（山東大學(xué)微生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室 濟(jì)南 250100）
劉穩(wěn)（山東大學(xué)微生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室 濟(jì)南 250100）
高培基（山東大學(xué)微生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室 濟(jì)南 250100）
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