SHEN Hong 1; 2; CHEN Mingjie 1; ZHAO Yongchang3; PAN Yingjie 41 Key Laboratory of Agricultural Genetics and Breeding of Shanghai; Institute of Edible Fungi; ShanghaiAcademy of Agricultural Sciences; Shanghai 201106; China; 2Microbiology Department; Nanjing Agriculture University; Nanjing; Jiangsu 201195; China; 3Yunnan Institute of Biological Technologies; Yunnan Agricultural Academy; Kunming; Yunnan 650205; China; 4Shanghai Fisheries University; Shanghai 200090; China
【中文摘要】 Fourteen Morchella samples (eleven from Yunnan and three from Zhejiang Provinces) were selected on the basis of differences in fruit body morphology. Ribosomal DNA internal transcribed spacers (ITS) were amplified in each case using the universal primer pair, ITS-1 and ITS-4, and the amplification products were purified and sequenced. Comparisons with sequence data in GenBank revealed that the 11 Morchella isolates collected from Yunnan belonged to four species: Morchella elata, Morchella conica...更多, Morchella crassipes and Morchella costata. The three isolates collected from Zhejiang Province (M12, M13 and M14) were designated as unknown Morchella species. When Verpa conica (AJ544206) (from the genus Verpa belonging to the same family as Morchella) was taken as the outgroup, the 14 isolates formed three groups, M. elata, M. costata (Group 1); Morchella esculenta, M. conica (Group 2); and M. crassipes, M12, M13 and M14 (Group 3). 還原
【英文摘要】 Fourteen Morchella samples (eleven from Yunnan and three from Zhejiang Provinces) were selected on the basis of differences in fruit body morphology. Ribosomal DNA internal transcribed spacers (ITS) were amplified in each case using the universal primer pair, ITS-1 and ITS-4, and the amplification products were purified and sequenced. Comparisons with sequence data in GenBank revealed that the 11 Morchella isolates collected from Yunnan belonged to four species: Morchella elata, Morchella conica, Morchella crassipes and Morchella costata. The three isolates collected from Zhejiang Province (M12, M13 and M14) were designated as unknown Morchella species. When Verpa conica (AJ544206) (from the genus Verpa belonging to the same family as Morchella) was taken as the outgroup, the 14 isolates formed three groups, M. elata, M. costata (Group 1); Morchella esculenta, M. conica (Group 2); and M. crassipes, M12, M13 and M14 (Group 3).
【英文關(guān)鍵詞】 Morchella; ITS sequences; Similarity coefficient
【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2007年02期 【DOI】CNKI:SUN:SYJB.0.2007-02-003