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  • Isolation, Purification and Chemical Structure of a Pleurotus tuber-regium Proteoglycan


    【發(fā)布日期】:2010-05-17

    WENG Bo-qi~ 1* ; JIANG Zhi-he~ 2 ; BIN Wen~ 3 ; WANG Yi-xiang~ 1 ; LUO Tao~ 2 ; LIN Yong~ 2 ~ 1 Agricultural Ecology Institute; Fujian Academy of Agricultural Sciences; Fuzhou; Fujian 350013; China; ~ 2 Research Center of Edible Fungi Development and Application; Fujian Academy of Agricultural Sciences; Fuzhou; Fujian 350013; China; ~ 3 Department of Pharmaceutics; Shenyang Pharmaceutical University; Shenyang ; Liaoning 110015; China

    【英文摘要】 A proteoglycan with a molecular mass of 39810 as determined by gel filtration, was obtained from dry, powdered sclerotia of Pleurotus tuber-regium following extraction with water, ethanol precipitation, protein removal by the Sevage method, dialysis, and Sephadex G-75 chromatography. Chemical analyses revealed the material to consist of 61.7% total sugar (calculated as glucose) and 38% nitrogen, and gas chromatography of acid-hydrolysis products indicated that the major monosaccharide components were glucose and galactose. Smith degradation data suggested that the main glycosidic linkage in the proteoglycan was β-1,4, and that β-1,3 linkages may also be present.

    【英文關(guān)鍵詞】 Chemical structure; Pleurotus tuber-regium; Proteoglycan; Sclerotia
    【基金】Sponsored by the Foundation of Fujian Province (2001Z128,2005N029)

    【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2006年03期 【DOI】CNKI:SUN:SYJB.0.2006-03-019

     
     
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