操玉平; 于敏; 沈業(yè)壽; 陸軍; 許寶奎
安徽大學(xué)生命科學(xué)學(xué)院安徽大學(xué)中藥研究與開(kāi)發(fā)重點(diǎn)實(shí)驗(yàn)室

【中文摘要】 培養(yǎng)大鼠胰島素瘤細(xì)胞(INS-1),以四氧嘧啶(AXN)損傷細(xì)胞,培養(yǎng)液中加入不同濃度的多糖AMP-1,檢測(cè)不同濃度AMP-1對(duì)INS-1細(xì)胞葡萄糖刺激的胰島素和C肽分泌量的影響,同時(shí)檢測(cè)細(xì)胞存活率。結(jié)果表明,一定濃度范圍AMP-1對(duì)AXN損傷的INS-1細(xì)胞分泌胰島素和C肽均具有一定的促進(jìn)作用,尤其是在葡萄糖刺激濃度為16.7mmol·L-1時(shí),效果顯著;AMP-1可減少AXN對(duì)INS-1細(xì)胞的損傷,使INS-1細(xì)胞存活率增加。

【英文摘要】 Graded concentrations of AMP-1 were added into the culture medium of INS-1 cells injured by alloxan. The amount of glucose-stimulated insulin and C-peptide secretions in different concentration of AMP-1 was determined and the survival rate was measured. The results showed that graded concentrations of AMP-1 could promote glucose-stimulated insulin and C-peptide secretions in INS-1 cells, especially in the concentration of 16.7 mmol·L -1 of glucose. AMP -1 could reduce the injuries induced by alloxan in INS-1 cells and the survival rate of cells increased significantly.

【中文關(guān)鍵詞】 蜜環(huán)菌; 菌索多糖; 四氧嘧啶; 胰島素瘤細(xì)胞; 胰島素; C肽
【英文關(guān)鍵詞】 Armillariella mellea; AMP; Alloxan; INS-1 cells; Insulin; C-peptide
【基金】國(guó)家自然科學(xué)基金項(xiàng)目(3040066);; 安徽省教育廳自然基金項(xiàng)目(2003kj021;2002kj017)。

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2009年01期 【DOI】CNKI:SUN:ZSYJ.0.2009-01-019