方春暉; 林志彬
北京大學(xué)醫(yī)學(xué)部藥理學(xué)系; 北京大學(xué)醫(yī)學(xué)部藥理學(xué)系 北京100083 ; 北京100083

【中文摘要】 本文初步考察巴氏蘑菇提取物 (ABE)對(duì)于小鼠離體脾淋巴細(xì)胞功能的影響。采用的方法包括正常和免疫受抑情況下脾淋巴細(xì)胞對(duì)于刀豆蛋白A刺激的增殖反應(yīng)和單雙向混合淋巴細(xì)胞反應(yīng)。結(jié)果發(fā)現(xiàn) ,在 5～ 10 0 μg/ml濃度范圍 ,ABE對(duì)小鼠離體脾淋巴細(xì)胞的增殖反應(yīng)具有增強(qiáng)作用 ;在 5～ 5 0 μg/ml濃度范圍 ,ABE可以部分恢復(fù)免疫受抑的小鼠離體脾淋巴細(xì)胞對(duì)刀豆蛋白A的應(yīng)答能力 ;在 10～ 5 0 0 μg/ml范圍內(nèi) ,ABE對(duì)單向和雙向混合淋巴細(xì)胞反應(yīng)都具有不同程度的增強(qiáng)作用 ,更高的濃度則具有抑制作用

【英文摘要】 We conducted a series of experiments to observe the effect of Agaricus blazei Murill.extract(ABE)on the functions of murine lymphocytes in vitro.The methods include splenocyte proliferation induced by ConA,using both normal murine lymphocytes and lymphocytes inhibited by cyclosporin A or hydrocortisone.mixed lymphocyte reaction was conducted using normal C57BL/6j lymphocytes and C57BL/6j lymphocytes pre-incubated with mitomycin C.We found that ABE(5～100μg/ml)can enhance the proliferation of murine lymphocytes stimulated by ConA in vitro,and can partially restore the response of inhabited lymphocyte to ConA within 5～50μg/ml.ABE(10～500μg/ml)cna also enhance mixed lymphocyte reaction.

【中文關(guān)鍵詞】 巴氏蘑菇; 淋巴細(xì)胞; 免疫藥理學(xué)
【英文關(guān)鍵詞】 Agaricus blazei Murill; Lymphocytes; Immunopharmacology

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2002年03期 【DOI】CNKI:SUN:ZSYJ.0.2002-03-018